2011

Complement C3 Deficiency Attenuates Chronic Hypoxia-Induced Pulmonary Hypertension in Mice

EM Bauer, H Zheng, S Comhair, S Erzurum… – PLoS ONE, 2011
… Recombinant human complement C3a (204881) and recombinant human complement C5a (234397) were from Calbiochem. Tissue factor antibody (SATF-IG) was from Affinity Biologicals. Chronic hypoxia-induced pulmonary hypertension. …

Low molecular weight heparin downregulates tissue factor expression and activity by modulating growth factor receptor-mediated induction of nuclear factor-κB

C Ettelaie, D Fountain, MEW Collier, AM ElKeeb… – Biochimica et Biophysica …, 2011
… To measure the total cellular TF antigen the cells were lysed in the presence of a protease inhibitor cocktail (Active Motif) and 20 μg of the samples analysed using a TF-antigen ELISA kit (Affinity Biologicals, Ancaster, Canada) as described before [27]. …

LJ Toltl, RC Austin… – Journal of Thrombosis and …, 2011 – Wiley Online Library
… Annexin V–fluorescein isothiocyanate (FITC) was from BD Biosciences (San Jose, CA, USA). Anti-tissue factor (TF)–FITC antibody was from Affinity Biologicals (Ancaster, ON).

LL Swystun, S Mukherjee, M Levine… – Journal of Thrombosis …, 2011 – Wiley Online Library
… City). Anti-human TF mAb maTF–fluorescein isothiocyanate (FITC) was from Affinity Biologicals (Ancaster, ON, Canada). …

MEW Collier… – Journal of Biological Chemistry, 2011 – ASBMB
… Total TF antigen within each isolated sample was measured using a TF EIA (Affinity Biologicals) as described previously (19). Activities of TF-containing and control …

2010

JL Yu, R Xing, C Milsom… – Thrombosis Research, 2010 – Elsevier
… Briefly, the single cell suspension was incubated with the sheep antibody against human TF (Affinity Biologicals Ancaster, ON, Canada) 

A Maraveyas, C Ettelaie, H Echrish… – Blood Coagulation & …, 2010 – journals.lww.com
… serum circulating-tissue factor antigen. The concentration of circulating-tissue factor antigen in the patients’ sera was measured using tissue factor-specific paired antibody ELISA method (Affinity Biologicals, Ancaster, Canada). …

2009

LL Swystun, LYY Shin, S Beaudin… – Journal of Thrombosis …, 2009 – Wiley Online Library
… Anti-human TF monoclonal antibody SATF was from Affinity Biologicals (Ancaster, ON, Canada), while HTF1 was from BD Pharmigen (San Jose …

DE Connor, T Exner, DDF Ma… – Blood Coagulation & …, 2009 – journals.lww.com
… Appropriately diluted antibody against human tissue factor (Affinity Biologicals Inc., Hamilton, Canada) was added to samples at concentrations specified in text to reverse the effect of tissue factor. …