Species Cross Reactivity

Species Cross Reactivity – Determined by Immunodiffusion
Each antibody listed has been tested for reactivity to the plasma of a variety of species. The double immunodiffusion (DID) technique used tests the ability of an antibody to precipitate an antigen in an agarose gel. The DID technique has limitations and a negative result should not necessarily be interpreted as non-recognition of an antigen. A number of antibodies may react with fewer recognition sites on the antigen of another species and be unable to form an insoluble immune complex (this is observed with many monoclonal antibodies). An antibody may demonstrate a greater spectrum of cross reactivity in applications such as immunoblotting and immunohistochemistry where antigen precipitation is not required. Below are examples of typical results seen in historical lots. See product insert for lot specific testing results. Species Cross-Reactivity – Using paired antibodies for EIAThe values given are the reactivities relative to normal human reference plasma when the test plasmas are assayed under the conditions specified in the protocol for each assay. Plasmas with reactivities of less than 1% are listed as negative. Optimization of the assay conditions may be required to efficiently utilize these assays for the measurement of these analytes in animal plasmas. Below are examples of typical results seen in historical lots. The only exception is the RBFG-EIA that uses normal rabbit plasma as a reference.
Antibodies to: Catalogue # Human Rat Mouse Rabbit Canine Porcine
Human Fibrinogen
SAFG-IG
***
 
SAFG-AP
***
*
*
*
*
 
Rabbit Fibrinogen
SARFG-IG
***
***
***
***
***
*
 
SARFG-AP
***
***
***
***
***
*
 
Human Prothrombin
SAFII-IG
***
*
 
SAFII-AP
***
*
*
*
*
*
 
Human Thrombin
SAHT-IG
***
*
*
Rabbit Thrombin
SART-IG
***
 
 
SART-AP
*
*
*
***
*
*
Bovine Thrombin
SABT-IG
 
SABT-AP
Human Factor V
SAFV-IG
*
 
SAFV-AP
*
 
Human von Willebrand Factor
GAVWF-IG
*
 
GAVWF-AP
*
Human Factor IX
SAFIX-IG
*
 
GAFIX-IG
*
SAFIX-AP
*
 
GAFIX-AP
***
Human Factor X
GAFX-IG
*
*
 
SAFX-AP
***
*
*
GAFX-AP
*
*
*
 
Human Factor XI
SAFXI-IG
*
GAFXI-IG
*
 
SAFXI-AP
*
GAFXI-AP
*
 
Human Factor XII
GAFXII-IG
*
 
GAFXII-AP
***
*
*
 
Human Factor XIII
SAFXIII-IG
***
*
*
*
*
Human Factor XIII-A (A Subunit)
SAF13A-AP
*
*
 
Human Prekallikrein
SAPK-IG
**
SAPK-AP
***
*
*
 
Human Kininogen
SAKN-IG
***
Human Protein C
SAPC-IG
*
*
 
GAPC-IG
*
SAPC-AP
*
 
GAPC-AP
*
Human Protein S
SAPS-IG
*
*
 
GAPS-IG
*
SAPS-AP
***
*
*
 
Human Plasminogen
SAPG-IG
***
***
***
***
***
***
GAPG-IG
***
***
***
***
***
***
 
SAPG-AP
***
***
***
***
***
***
GAPG-AP
***
***
***
***
***
***
 
Human Antithrombin (ATIII)
SAAT-IG
***
GAAT-IG
***
*
*
 
SAAT-AP
***
***
***
*
***
***
GAAT-AP
***
***
***
***
***
 
 
Human Heparin Co-Factor II
SAHC2-IG
***
***
***
***
***
*
GAHC2-IG
***
***
***
***
***
***
 
GAHC2-AP
***
***
***
***
***
***
Human Alpha 2-Antiplasmin
SA2AP-IG
***
 
GA2AP-IG
***
GA2AP-AP
***
 
Human Alpha 1-Antitrypsin
SA1AT-IG
***
GA1AT-IG
***
 
SA1AT-AP
***
GA1AT-AP
***
 
Human Alpha 2-Macroglobulin
GAA2M-IG
***
***
***
***
GAA2M-AP
***
*
***
***
***
 
Human C1-Inhibitor
GACINH-IG
***
GACINH-AP
***
 
Human Beta 2-Glycoprotein-1 (APO-H)
GAB2G-IG
***
***
***
***
GAB2G-AP
***
***
*
***
***
 
Human Vitronectin
SAVN-IG
**
 
SAVN-AP
***
*
*
*
*
*
 
Legend:
*** strong positive
* weak positive
— negative
NT not tested
Catalogue No. Analyte Human Rat Mouse Rabbit Canine Porcine
FG-EIA Fibrinogen antigen
100%
17%
7%
8%
neg
5%
 
RBFG-EIA Rabbit Fibrinogen antigen
40%
5%
7%
100%
18%
4%
 
FII-EIA Prothrombin antigen
100%
neg
neg
neg
neg
neg
 
 
FV-EIA Factor V antigen
100%
neg
neg
40%
neg
26%
 
FVII-EIA Factor VII antigen
100%
neg
neg
8%
neg
4%
 
 
F8C-EIA Factor VIII antigen
100%
neg
neg
18%
neg
50%
 
VWF-EIA von Willebrand Factor antigen
100%
4%
neg
25%
9%
34%
 
 
FIX-EIA Factor IX antigen
100%
neg
neg
8%
neg
7%
 
 
FX-EIA Factor X antigen
100%
neg
3%
4%
neg
1%
 
 
FXI-EIA Factor XI antigen
100%
5%
4%
77%
6%
12%
 
 
FXII-EIA Factor XII antigen
100%
neg
neg
5%
neg
neg
 
KN-EIA Kininogen antigen
100%
neg
neg
4%
neg
neg
 
 
PK-EIA Prekallikrein antigen
100%
neg
neg
5%
neg
neg
 
FXIII-EIA Factor XIII antigen
100%
neg
neg
10%
neg
neg
 
PC-EIA Protein C antigen
100%
neg
neg
neg
neg
neg
 
 
PCI-EIA Protein C Inhibitor antigen
100%
neg
neg
6%
neg
neg
 
PS-EIA Protein S antigen
100%
neg
neg
14%
5%
3%
 
 
ATIII-EIA Antithrombin antigen
100%
14%
23%
7%
11%
4%
 
HCII-EIA Heparin Cofactor II antigen
100%
1%
1%
5%
2%
neg
 
 
APOH-EIA β2-Glycoprotein-1 (Apolipoprotein-H, APO-H) antigen
100%
7%
neg
2%
11%
7%
 
 
PG-EIA Plasminogen antigen
100%
neg
neg
2%
8%
neg
 
 
Legend:
neg=negative
NT=not tested