Kininogen (Fitzgerald Factor)(HMWK & LMWK), human

Antibody	Host	Catalogue No.	Size	Product Insert
Purified IgG	Sheep	SAKN-IG	10 mg
Affinity purified IgG	Sheep	SAKN-AP	0.5 mg
Affinity purified, peroxidase conjugated IgG	Sheep	SAKN-APHRP	0.2 mg
Matched Pair Antibodies for EIA	Sheep	KN-EIA	4 plates

Description of Kininogen

Kininogens are multi-function proteins that are involved in the processes of coagulation, anticoagulation, fibrinolysis, inflammation and cell adhesion. Kininogens are produced in the liver but have also been found in platelets, granulocytes, renal tubular cells and skin. Two forms of kininogen are identified in plasma, both of which are the result of differential splicing of a single gene. High molecular weight kininogen (HK), previously known as Fitzgerald Factor, is a single chain glycoprotein of 120 kDa with a plasma concentration of 80 μg/mL (660 nM). Low molecular weight kininogen (LK), also known as α-cysteine protease inhibitor, is a single chain glycoprotein of 68 kDa with a plasma concentration of 160 μg/mL (2.35 μM). HK and LK share a common heavy chain and bradykinin domain, but have unique light chains. It is the light chain of HK that is responsible for the coagulant cofactor activity by binding to anionic surfaces and for the ability to bind the zymogens prekallikrein (PK) and factor XI (FXI). HK is cleaved by kallikrein in several sequential steps that result in the release of a potent vasodilator bradykinin and the conversion to a two-chain form of HK with increased cofactor activity. In plasma, most of the PK and FXI circulate in complex with HK. Activation of PK by FXIIa generates kallikrein, which initiates reciprocal activation of PK and FXI. The presence of HK also serves to protect kallikrein and activated FXI from protease inhibitors such as C1-Inhibitor, but regulation of the system may be accomplished through proteolytic inactivation of the HK cofactor activity by these enzymes.^1-2

References:

1. Coleman RW, Schmaier AH; Contact System: A Vascular Biology Modulator With Anticoagulant, Profibrinolytic, Antiadhesive and Proinflammatory Attributes. Blood 90, pp 3819-3843, 1997.

2. DeLa Cadena R, Watchtfogel YT, Colman RW, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 219-240, J.B. Lippincott Co., Philadelphia, 1994.

Related Products:

SAKN-IG Sheep anti-human Kininogen, whole IgG from antiserum

SAKN-HRP Sheep anti-human Kininogen, peroxidase-labelled IgG

SAKN-AP Sheep anti-human Kininogen, affinity purified IgG

KN-DP Human plasma deficient in Kininogen, immune depleted, Frozen

KN-EIA Paired antibody set for ELISA of Kininogen, 4 x 96 wells

KN-LDP Human plasma deficient in Kininogen, immune depleted, Lyophilized