2011

Long-term expression of human coagulation factor VIII in a tolerant mouse model using the phiC31 integrase system

CL Chavez, A Keravala, JN Chu, AP Farruggio… – Human Gene Therapy
… manufacturer’s protocol. hFVIII concentration was determined using the Matched-Pair Antibody Set for ELISA of hFVIII antigen, following the manufacturer’s protocol from Affinity Biologicals (Ancaster, Ontario, Canada). FVIII inhibitor antibodies were …

Bioengineering of Coagulation Factor VIII for Efficient Expression Through Elimination of a Dispensable Disulfide Loop

SR Selvaraj, AN Scheller, HZ Miao, RJ Kaufman… – Journal of Thrombosis and …, 2011
… were purchased from Gibco BRL (Gaithersburg, MD). COAMATIC was purchased from DiaPharma (West Chester, OH). F8C-EIA was purchased from Affinity Biologicals Inc. Page 6. (ON, Canada). FuGENE-6 transfection reagent and restriction enzymes were purchased …

von Willebrand Disease in Children: Diagnosis and Management of a Pediatric Cohort in One Single Center in Argentina

M Bonduel, JP Frontroth, M Hepner, G Sciuccati… – Semin Thromb Hemost, 2011
… horseradish peroxidase conjugated antibody against FVIII (SAF8C-HRP; Affinity Biologicals, Ontario, Canada) for detection. …

Cleavage of von Willebrand Factor by Granzyme M Destroys Its Factor VIII Binding Capacity

MJ Hollestelle, KW Lai, M van Deuren, PJ Lenting… – PLoS ONE, 2011
… Samples were resolved in Laemmli sample buffer and subjected to SDS-PAGE (7.5%) and Western blotting, using polyclonal antibodies against VWF (Dako Glostrup, Denmark) and FVIII (Affinity Biologicals NC). Plasma measurements in patients with meningococcal disease. …

p. Tyr365Cys change in factor VIII: haemophilia A, but not as we know it

AE Bowyer, A Goodeve, R Liesner, AD Mumford… – British Journal of Haematology
… All clotting and chromogenic tests were performed on Sysmex CA7000 or CA1500 instrumentation (Kobe, Japan). FVIII antigen (FVIII:Ag) was determined by enzyme-linked immunosorbent assay with the Visualize FVIII antigen kit (Affinity Biologicals, Ancaster, ON, Canada). …

MP Kolind, PL Norby, MW Berchtold… – Journal of biotechnology, 2011 – Elsevier
… Paisley, UK), MTX (Wyeth, Taplow, UK), HiSpeed plasmid Maxi kit (Qiagen, Hilden, Germany), restriction enzymes (New England Biolabs, Hitchin, UK), pTT5 vector (National Research Council, Montreal, Canada), F8C-EIA FVIII ELISA kit (Affinity Biologicals, ON, Canada …

2010

VR Arruda, HH Stedman, V Haurigot… – …, 2010 – bloodjournal.hematologylibrary.org
… determined as previously described. 27 Canine FIX (cFIX) antigen concentration was
determined by enzyme-linked immunosorbent assay (ELISA) using matched-pair
antibodies to cFIX (Affinity Biologicals). FIX clotting activity was …

TK Greene, C Wang, JD Hirsch… – …, 2010 – bloodjournal.hematologylibrary.org
… supernatant. Percent positive cells for hF8 were determined using a sheep polyclonal
anti-hF8 fluorescein isothiocyanate (FITC)–labeled antibody (Affinity Biologicals) and
flow cytometry after intracellular fixation and permeabilization. ..

I Liao – Biomaterials, 2010 – Elsevier
… differentiate into skeletal muscle. The engineering of myoblasts to produce FVIII was
performed by screening for positive clones with supernatant secretion of canine FVIII
via ELISA (Affinity Biologicals). The medium were supplemented …

MP Kolind, PL Nørby, TV Flintegaard… – Journal of …, 2010 – Elsevier
… New England Biolabs), pCR ® -Blunt II-TOPO ® vector (Invitrogen), pTT5 vector (National Research Council Canada), Fectin293 (Invitrogen), HEK293 cells (FreeStyle HEK-293-F cells), FreeStyle Medium, DMEM (Invitrogen) FVIII ELISA kit (Affinity Biologicals) …

D Green, N Foiles, C Chan, J Kang, PJ Schreiner… – Stroke, 2010 – Am Heart Assoc
… FVIII antigen was assayed in EDTA plasma using the VisuLize FVIII Antigen Kit (Affinity Biologicals), which uses a double-antibody ELISA. Von Willebrand factor (vWF) antigen was …

R Gheisari, B Bomke, T Hoffmann… – Hämostaseologie, 2010 – schattauer.de
… FVIII : Ag was determined by ELISA (Affinity Biologicals, Ancaster, ON, Canada) accordingly to the manufacture’s instructions. Mixing studies with patient and normal plasma …

A Thakur, R Sengupta, H Matsui… – … Research Part B: …, 2010 – Wiley Online Library
… ELISA assay for cFVIII measurement. Canine FVIII secretion was quantified using a cFVIII-specific ELISA kit (Affinity Biologicals, Hamilton, Canada), using normal pooled canine plasma (Central Animal Facilities, McMaster University) to generate the standard curves. RESULTS. …

MB Irigoyen, L Primiani, M Felippo, M Candela… – …, 2010 – Wiley Online Library
… Preparations were stable at least for 1 year. rFVIII binding to microspheres was checked by adding to 2.5 μL of each FVIII-m and control-m, 5 μL of a 1/500 dilution of biotinylated sheep IgG anti-human factor VIII (Affinity Biologicals Inc., Ancaster, Ontario, Canada). …

W Xu, J Chen, G Yamasaki, JE Murphy… – Molecular biotechnology, 2010 – Springer
… FVIII ELISA Assay The FVIII protein concentration in TCF was estimated by a FVIII
ELISA. The ELISA assay was performed by using a matched-pair antibody set for
human FVIII (Cat# F8C- EIA, Affinity Biologicals Inc.). Briefly …

NC Josephson – US Patent App. 20,100/233,119, 2010 – freepatentsonline.com
… FYIII Protein Expression. FVIII protein production by transduced fibroblasts was measured in culture media with an ELISA kit per manufacturer’s instructions (Matched-Pair Antibody Set for ELISA of hFVIII antigen, Affinity Biologicals, INC, Ancaster, ON, Canada). …

S Bang, L Thim… – US Patent App. 20,100/ …, 2010 – freepatentsonline.com
… FVIII concentration in mU/ml. ELISA Assay. Factor VIII antigen was determined using
the commercial ELISA kit from Affinity Biologicals (VisuLize Factor VIII Antigen Kit,
Lot AG8-0006) essentially as described by the manufacturer. …

J Sivalingam, S Krishnan, WH Ng, SS Lee… – Molecular …, 2010 – nature.com
… Sweden) and the recommended protocol. Human-specific FVIII levels were
quantitated in citrated mouse plasma using an ELISA kit (Affinity Biologicals, Ancaster, Ontario, Canada). Assessment of phenotypic correction was …

SS Dhadwar, J Kiernan, J Wen… – Journal of Thrombosis …, 2010 – Wiley Online Library
… mice. Briefly, wells were coated first with goat anti-canine FVIII capture antibody
(Affinity Biologicals), followed by normal canine plasma and finally mouse plasma
samples

HJ Jeon, TK Oh, OH Kim… – Yonsei Medical Journal, 2010 – ncbi.nlm.nih.gov
… The plasma concentration of human FVIII was measured by ELISA as described by the manufacturer (Affinity Biologicals, Hamilton, ON, …

2009

JH Foley… – Journal of Thrombosis and …, 2009 – Wiley Online Library
… the Kingston General Hospital (KGH) in Kingston, Ontario, Canada, and FVIII-deficient plasma (FVIII-DP) was purchased from Affinity Biologicals, Inc …

L Liu, H Liu, C Mah… – Gene therapy, 2009 – nature.com
… prepared and stained with antibodies against FVIII (polyclonal, affinity-purified, sheep anti-human FVIII at 1:500 dilution, from Affinity Biologicals Inc., Ancaster …