VisuLize Factor VII ELISA Kit

VisuLize Factor VII Antigen ELISA Kit

FVII, F7, Factor VII, coagulation ELISA kit Factor VII (FVII, also known as Stable Factor and Proconvertin) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FVII is normally ~0.5 μg/ml (10 nM) in plasma. In its zymogen form FVII is a single chain molecule of ~50 kDa. It contains two EGF-like domains and an amino-terminal domain containing 10 γ-carboxyglutamic acid (Gla) residues. These Gla residues allow FVII to bind divalent metal ions and participate in calcium-dependent binding interactions. FVII and activated FVII (FVIIa) bind to tissue factor exposed at the site of vascular injury. FIXa, FXa or FVIIa rapidly activate tissue factor-bound FVII to FVIIa in the presence of calcium and phospholipid. Thrombin and FXIIa are able to activate FVII in the fluid phase in the absence of cofactors. The activation of the single chain zymogen FVII occurs by proteolysis after residue Arg152, resulting in a two-chain active serine protease consisting of a 30 kDa heavy chain and a 18 kDa light chain. In complex with tissue factor, phospholipid and calcium, FVIIa is able to activate F.X and F.IX. Free FVIIa in plasma is remarkably stable, but the activity of FVIIa/TF complex is regulated by Tissue Factor Pathway Inhibitor (TFPI) in the presence of F.Xa, and also by Antithrombin (ATIII) in the presence of heparin1-3.   References:   1. Rao LVM, Bajaj SP, Rapaport SI; Activation of Human Factor VII During Clotting in Vitro; Blood 65, pp 218-226, 1985.   2. Lawson, JH, Butenas S, Ribarik N, Mann KG; Complex-dependent Inhibition of Factor VIIa by Antithrombin III and Heparin; JBC 268 pp 767- 770, 1993.   3. Nemerson Y, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 81-93, J.B. Lippincott Co., Philadelphia PA, USA, 1994.   VisuLize Factor VII Antigen ELISA kit is for Research Use Only and must not be used for diagnostic applications.     Features:
  • Rapid sandwich ELISA to measure Factor VII antigen (FVII:Ag)
  • Detection limit of <0.01 IU/mL (<1 %) FVII antigen
  • Rapid sandwich ELISA to measure Factor VII antigen (FVII:Ag)
  • 2 hour total incubation time
  • FVII:Ag reported as IU/ml traceable to ISTH SSC standard for FVII activity
  • Includes normal and low control
  • Intra-assay precision: 4.92 %
Applications:
  •  The VisuLize FVII Antigen kit is an Enzyme Immunoassay for the quantitative determination of Factor VII antigen in human plasma samples using the double antibody enzyme linked immuno-sorbent assay (ELISA).
Kit Contents:
  • Pre-coated plate in a convenient 96 well; 6×16 strip format
  • Calibrator plasma, Control A and Control B plasmas (2 vials of each)
  • Sample diluent and wash buffer concentrate
  • Ready-to-use detecting antibody, TMB substrate and stop solutions
 
Product Catalogue No. Size Product Insert
VisuLize FVII Antigen ELISA Kit FVII-AG 1 plate
 

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ELISA Kits: FVIII-AG – VisuLize FVIII Antigen Kit, FIX-AG – VisuLize FIX Antigen Kit, TAFI-AG – VisuLize TAFI Antigen Kit, FXI-AG VisuLize FXI Antigen Kit   Antibodies: SAFVII-IG – Sheep anti-human FVII, whole IgG from serum, 10mg vial; SAFVII-AP – Sheep anti-human FVII, affinity purified IgG, 0.5mg vial; SAFVII-HRP – Sheep anti-human FXI, peroxidase conjugated IgG, 0.2mg vial; FVII-EIA – matched pair antibodies for ELISA of human FVII, sufficient reagents for 4×96 well plate   Plasma: FVII-DP – Factor XI deficeint plasma – citrated plasma, immune depleted of FVII, 1ml to bulk; INH11-DP – Factor XI Inhibitor Plasma, available in various activities (recorded as BU/mL) 1ml to bulk; INH11-LDP – lyophilized Factor XI Inhibitor plasma, available in various activities (recorded as BU/mL) 1ml to bulk   Diagnostic Deficient Plasma: FRDP070125 – VisuDep-F Frozen Factor VII Deficient Plasma