Factor IX, human

Antibody	Host	Catalogue No.	Size	Product Insert
Purified IgG	Goat	GAFIX-IG	10 mg
Affinity purified IgG	Goat	GAFIX-AP	0.5 mg
Peroxidase conjugated IgG	Goat	GAFIX-HRP	0.2 mg
Affinity purified, peroxidase conj. IgG	Goat	GAFIX-APHRP	0.1 mg
Affinity purified, FITC conjugated IgG	Goat	GAFIX-APFTC	0.1 mg
Affinity purified, biotinylated IgG	Goat	GAFIX-APBIO	0.1 mg
Purified IgG	Sheep	SAFIX-IG	10 mg
Affinity purified IgG	Sheep	SAFIX-AP	0.5 mg
Affinity purified, peroxidase conj. IgG	Sheep	SAFIX-APHRP	0.1 mg
Matched Pair Antibodies for EIA		FIX-EIA	5 plates

Factor IX, canine

Antibody	Host	Catalogue No.	Size	Product Insert
Matched Pair Antibodies for EIA		CFIX-EIA	4 plates

Description of Factor IX (FIX)

Factor IX (FIX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of FIX is normally around 5 μg/ml (87 nM) in plasma. The biological importance of FIX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity, normal antigen) defect in FIX function.

In its proenzyme or zymogen form FIX is a single chain molecule of 55,000 daltons. It contains two EGF-like domains and an amino-terminal domain containing 12 γ-carboxy-glutamic acid (Gla) residues. These Gla residues allow FIX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXIa) and/or by a complex of VIIa/tissue factor/phospholipid and activated Factor X between residues Arg146-Ala147 and between Arg180-Val181. The terminal activated product in either case is FIXaβ, a two-chain enzyme consisting of a heavy chain (28,000 daltons), a light chain (18,000 daltons) and an activation peptide product of 11,000 daltons. FIX can also be cleaved into inactive products by thrombin and by elastase.

The activity of FIXaβ in plasma is inhibited by antithrombin and this inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin ^1-3.

References:

1. Lawson, JH, Mann KG; Cooperative Activation of Human F.IX by the Human Extrinsic Pathway of Coagulation; JBC 266 pp11317-11327, 10991.

2. Enfield DL, Thompson AR; Cleavage and Activation of Factor IX by Serine Proteases; Blood 64, pp 821-831, 1984.

3. Limentani SA, Furie BC, Furie B, in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 94-108, J.B. Lippincott Co., Philadelphia PA, USA, 1994

Related Products:

GAFIX-AP Goat anti-human F.IX, affinity purified IgG

GAFIX-HRP Goat anti-human F.IX, IgG-peroxidase

GAFIX-APHRP Goat anti-human F.IX, APIgG-peroxidase

SAFIX-IG Sheep anti-human F.IX, IgG from antiserum

SAFIX-AP Sheep anti-human F.IX, affinity purified IgG

SAFIX-APHRP Sheep anti-human F.IX, APIgG-peroxidase

FIX-EIA Paired antibody set for ELISA of F.IX, 5 x 96 wells

FIX-DP Human plasma deficient in Factor IX, immune depleted

FIX-AG VisuLize™ FIX Antigen ELISA Kit

INH9-DP Factor IX Inhibitor Plasma, Frozen

INH9-LDP Factor IX Inhibitor Plasma, Lyophilized