Factor FIX – Published Papers Using Affinity Biologicals Products

2011

Oral Administration Of Therapeutic Agent Coupled To Transporting Agent

G Hortelano… – US Patent 20,110,301,097, 2011
… Adult immunocompetent C57BL/6 mice (20 weeks of age) were each fed 100 μg of DNA nanoparticles orally (n=3). Mice were bled regularly, and the concentration of hFIX in plasma determined by ELISA (Affinity Biologicals). …

Methods and Compositions for use in Gene Therapy for Treatment of Hemophilia

KA High… – US Patent 20,110,263,690, 2011
… for 1 hour. Tissue sections were incubated overnight in the presence of an affinity
purified goat anti-human F.IX antibody (Affinity Biologicals, Hamilton, Ontario, Canada)that was diluted 1:1000 in PBS/1% BSA. After three washes …

Engineered factor IX variants bypass FVIII and correct hemophilia A phenotype in mice.

P Milanov, L Ivanciu, D Abriss, P Quade-Lyssy… – Blood, 2011
… St Louis, MO) was used as a capture antibody. Peroxidase-conjugated polyclonal goat anti–hFIX (Affinity Biologicals, Hamilton, ON) was used as the detecting antibody 13. For visualization of activated or not activated FIX heavy chain, 300 ng protein of either wild type …

HUMAN FACTOR IX VARIANTS WITH AN EXTENDED HALF LIFE

DW Stafford, DM Mann… – US Patent 20,110,154,516, 2011
… The amount of FIX remaining in the circulation was measured by ELISA using wild type FIX as a standard. Antibodies for the ELISA were obtained from Affinity Biologicals (Product numbers SAFIX-AP SAFIX-APHRP). The curve was fit to one exponential decay. …


Transgene loss and changes in the promoter methylation status as determinants for expression duration in non-viral gene transfer for factor IX

J Schuettrumpf, P Milanov, D Abriss… – Human gene …, 2011 – liebertonline.com … Sigma-Aldrich, St. Louis, MO) was used as a capture antibody. Peroxidase-conjugated polyclonal goat anti-human FIX (Affinity Biologicals, Hamilton, ON, Canada) was used as the detecting antibody. FIX functional activity was …

2010

VR Arruda, HH Stedman, V Haurigot… – …, 2010 – bloodjournal.hematologylibrary.org
… determined as previously described. 27 Canine FIX (cFIX) antigen concentration was
determined by enzyme-linked immunosorbent assay (ELISA) using matched-pair
antibodies to cFIX (Affinity Biologicals). FIX clotting activity was …

A Ishiwata, J Mimuro, H Mizukami… – Thrombosis …, 2010 – Elsevier
… Monoclonal antibody 3A6-bound human FIX or macaque FIX was detected with a horseradish peroxidase (HRP)-conjugated goat anti-human FIX polyclonal antibody (Affinity Biologicals Inc., Hamilton, Ontario, Canada). Purified human FIX was used as a standard. …

CL Chavez, A Keravala, LE Woodard… – Human gene …, 2010 – liebertonline.com
… no. 11-867-423-001; Roche), goat anti-hFIX (GAFIX-AP; Affinity Biologicals, Ancaster, ON, Canada)…

G Zhang, Q Shi, SA Fahs… – …, 2010 – bloodjournal.hematologylibrary.org
… An affinity-purified horseradish peroxidase-conjugated goat-anti-hFIX polyclonal
antibody (Affinity Biologicals) was used as a detecting antibody.

IH Lim, JS Kim, G Lee, M Choi… – Cells and Culture, 2010 – Springer
… C. Cell concentration dur- ing culture was measured using a hemocytometer by
tryphan blue exclusion method. The secreted rhFIX protein was determined using
sandwich ELISA assay (Affinity Biologicals, Ontario, Canada). …

Q Cheng, EI Tucker, MS Pine… – …, 2010 – bloodjournal.hematologylibrary.org
… Samples were size-fractionated on 10% nonreducing SDS-polyacrylamide gels and transferred to polyvinylidene fluoride membranes. Chemiluminescent Western blotting was performed using goat anti-human fIX IgG (Affinity Biologicals). HK-fXI binding assay. …

EC Murphy, M Morgelin, JC Cooney… – Microbiology, 2011 – Soc General Microbiol
… goat anti-FXI from Affinity Biologicals Inc. …

JM Wilson… – EP Patent 1,696,036, 2010 – freepatentsonline.com
… serum albumin (BSA) for 1 hour. Sections were subsequently incubated overnight
with an affinity purified goat anti-human F.IX antibody (Affinity Biologicals) that was
diluted 1:1000 in PBS/1% BSA. After three washes (10 minutes …

D Verma, B Moghimi, PA LoDuca… – Proceedings of the …, 2010 – National Acad Sciences
… Sections were blocked with 2% donkey serum in PBS for 30 min. Goat α-hF.IX (1:400; Affinity Biologicals) …

V Haurigot, F Mingozzi, G Buchlis, DJ Hui… – Molecular …, 2010 – nature.com
… cFIX antigen detection. cFIX antigen levels were measured by immunoassay using a polyclonal matched-pair antibody set for cFIX enzyme-linked immunosorbent assay (Affinity Biologicals, Ancaster, Ontario, Canada) following manufacturer’s instructions. …

UE Fima… – US Patent App. 12/826,754, 2010 – Google Patents
… [0017] FIG. 3 A is a bar graph showing harvests of limited diluted transfected and selected cells with FIX-CTP and FIX- CTP-CTP variants in the presence of 5 (xg/ml ofVitamin K3. The level of FIX was quantified using Human FIX ELISA kit (Affinity Biologicals; cat. No. …

2009

R Hartmann, M Dockal… – … of Thrombosis and …, 2009 – Wiley Online Library
… an anti-FIX enzyme-linked immunosorbent assay, using affinity-purified sheep anti-human FIX IgG (SAFIX-AP; Affinity Biologicals, Ancaster, ON …

R Asselta, V Rimoldi, I Guella… – …, 2010 – bloodjournal.hematologylibrary.org
… GAFXI-IG) from Affinity Biologicals, previously adsorbed for 40 minutes at room temperature to 14 µL of magnetic bead-protein G (Dynal Biotech), and incubated …

ENTIVIRAL VECTORS ENCODING CLOTTING FACTORS FOR GENE THERAPY

JG Mcarthur, DJ Talbot, AD Simmons… – EP Patent …, 2009 – freepatentsonline.com
… 5X with BBST and incubated with HRP conjugated goat anti-human factor IX antibody (1:100) (Affinity Biologicals, Hamilton, Canada) for …