Antithrombin – ATIII, human

Antibody	Host	Catalogue No.	Size	Product Insert
purified IgG	Goat	GAAT-IG	10 mg
affinity purified IgG	Goat	GAAT-AP	0.5 mg
purified IgG	Sheep	SAAT-IG	10 mg
affinity purified IgG	Sheep	SAAT-AP	0.5 mg
affinity purified, peroxidase conj. IgG	Sheep	SAAT-APHRP	0.1 mg
affinity purified, FITC conjugated IgG	Sheep	SAAT-APFTC	0.1 mg
affinity purified, biotinylated IgG	Sheep	SAAT-APBIO	0.1 mg
Matched Pair Antibodies
- For EIA of Antithrombin		ATIII-EIA	5 plates
- For EIA of T-AT Complex		TAT-EIA	5 plates
Matched Pair Inhibitor and Control Antibodies
- For Neutralization of Antithrombin		AT-NU10	10 det.

Antithrombin (ATIII), rabbit

Antibody	Host	Catalogue No.	Size	Product Insert
purified IgG	Sheep	SARAT-IG	10 mg
affinity purified IgG	Sheep	SARAT-AP	0.5 mg
purified IgG	Sheep	SARAT-HRP	0.2 mg

Antithrombin (ATIII), murine

Antibody	Host	Catalogue No.	Size	Product Insert
Matched Pair Antibodies for EIA		MATIII-EIA	4 plates

Description of Antithrombin (ATIII)

Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 μg/ml (~3.5 μM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa^1-3.

References:

1. Damus PS, Rosenberg RD; Antithrombin – Heparin Cofactor; Methods in Enzymology 45, pp 653-669, 1976.

2. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982.

3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

Related Products:

GAAT-AP Goat anti-ATIII, affinity-purified IgG

SAAT-IG Sheep anti-ATIII, whole IgG from antiserum

SAAT-AP Sheep anti-ATIII, affinity-purified IgG

SAAT-APHRP Sheep anti-ATIII, APIgG-peroxidase

ATIII-EIA Paired antibody set for ELISA of ATIII, 5 x 96 wells

TAT-EIA Paired antibody ELISA of Thrombin-ATIII complexes

AT-NU10 Paired antibody set for ATIII neutralization in plasma

ATIII-DP Human plasma deficient in ATIII, immune depleted, Frozen

ATIII-LDP Human plasma deficient in ATIII, immune depleted, Lyophilized

ATHC-DP Human ATIII & HCII deficient plasma, immune depleted, Frozen

ATHC-LDP Human ATIII & HCII deficient plasma, immune depleted, Lyophilized